<br><font size=2 face="sans-serif">Young,</font>
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<br><font size=2 face="sans-serif">Assuming you made 8 bit grayscale tiff files from the RGB images, perhaps there are no 255 values in the volume. Try a lower iso value....john</font>
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<td><font size=6 color=red face="Times New Roman"><b>Internet Mail Message</b></font>
<br><font size=2 color=red face="sans-serif">Received from host: </font>
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<td><font size=1 face="sans-serif"><b>"Young" <ytsemikim@hotmail.com></b></font>
<br><font size=1 face="sans-serif"> Sent by: vtkusers-admin@public.kitware.com</font>
<p><font size=1 face="sans-serif">01/08/2003 06:07 PM</font>
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<br><font size=1 face="sans-serif"> To: <vtkusers@public.kitware.com></font>
<br><font size=1 face="sans-serif"> cc: (bcc: John Anast-JM/PGI)</font>
<br><font size=1 face="sans-serif"> Subject: [vtkusers] Reconstruction with 2D tiff images</font></table>
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<br><font size=2 face="Arial">Hi, vtk users.</font>
<br><font size=2 face="Arial">I have a question in terms of 3D reconstruction using 2d tiff images. </font>
<br><font size=2 face="Arial">I have 30 serial rat brain section images(RGB) (from cortex to striatum) taken by digital camera and saved into Tiff file (1392x1040). Then I resize images to 500x374 and rename them (Hfm.01, Hfm.02-----Hfm.30). I tried to load these 30 images and reconstruct a 3D structure. This is Tcl code what I used. </font>
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<br><font size=2 face="Arial">package require vtk</font>
<br><font size=2 face="Arial">package require vtkinteraction</font>
<br><font size=3 face="Times New Roman"> </font>
<br><font size=2 face="Arial">vtkTIFFReader reader</font>
<br><font size=2 face="Arial">reader SetFilePrefix "D:/.../.../Hfm"</font>
<br><font size=2 face="Arial">reader SetDataByteOrderToLittleEndian</font>
<br><font size=2 face="Arial">reader SetDataExtent 0 499 0 373 1 30</font>
<br><font size=2 face="Arial">reader SetDataOrigin 0.0 0.0 0.0</font>
<br><font size=2 face="Arial">reader SetDataSpacing 1 1 3</font>
<br><font size=2 face="Arial">reader SetDataMask 0x7fff</font>
<br><font size=2 face="Arial">reader Update</font>
<br><font size=3 face="Times New Roman"> </font>
<br><font size=2 face="Arial">vtkMarchingCubes iso</font>
<br><font size=2 face="Arial">iso SetInput [reader GetOutput]</font>
<br><font size=2 face="Arial">iso SetValue 0 255</font>
<br><font size=3 face="Times New Roman"> </font>
<br><font size=2 face="Arial">vtkPolyDataMapper isoMapper</font>
<br><font size=2 face="Arial">isoMapper SetInput [iso GetOutput]</font>
<br><font size=2 face="Arial">isoMapper ScalarVisibilityOff</font>
<br><font size=3 face="Times New Roman"> </font>
<br><font size=2 face="Arial">vtkActor isoActor</font>
<br><font size=2 face="Arial">isoActor SetMapper isoMapper</font>
<br><font size=2 face="Arial">eval [isoActor GetProperty] SetColor 1 1 1</font>
<br><font size=3 face="Times New Roman"> </font>
<br><font size=2 face="Arial">vtkRenderer ren1</font>
<br><font size=2 face="Arial">vtkRenderWindow renWin</font>
<br><font size=2 face="Arial">renWin AddRenderer ren1</font>
<br><font size=2 face="Arial">vtkRenderWindowInteractor iren</font>
<br><font size=2 face="Arial">iren SetRenderWindow renWin</font>
<br><font size=2 face="Arial">ren1 AddActor isoActor</font>
<br><font size=2 face="Arial">ren1 SetBackground 0.1 0.2 0.4</font>
<br><font size=2 face="Arial">renWin SetSize 500 500</font>
<br><font size=2 face="Arial">renWin Render</font>
<br><font size=3 face="Times New Roman"> </font>
<br><font size=2 face="Arial">When I render, I only see just background in rendering window. There is noting in my rendering window. Since I build my own vtk using CMake, I have no problem for using Patented fuction...</font>
<br><font size=2 face="Arial">Is there any body who can help me.... Please.</font>
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