<div>Hi Flory,</div>
<div> </div>
<div>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">Thanks Flory. I used this code and the problem with vtkTIFFReader is solved. I haven't got any Error. </font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">vtkTIFFReader reader = new vtkTIFFReader();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">reader.SetFileDimensionality(2);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">reader.SetFilePrefix("C:\\Data\\3Daxial\\t8a");</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">reader.SetFilePattern("%s%02d.tif");</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">reader.SetFileNameSliceOffset(0);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">reader.SetDataExtent(0, 512, 0, 512, 0, 27);<span style="mso-spacerun: yes"> </span></font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">reader.SetDataSpacing(0.1, 0.1, 0.5);<span style="mso-spacerun: yes"> </span></font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">reader.SetDataOrigin(0.0, 0.0, 0.0);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">reader.Update();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">But I still have a problem with the rendering. I don't see of my data in the rendering window. <span style="mso-spacerun: yes"> </span>I see only some strange polygons and I don't Know what's the fault.</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">This is my Java Code.</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">vtkImageGaussianSmooth gsmooth = new vtkImageGaussianSmooth();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>gsmooth.SetInput(reader.GetOutput());</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>gsmooth.SetStandardDeviation(sdev[0], sdev[1], sdev[2]);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>gsmooth.SetRadiusFactors(radius[0], radius[1], radius[2]);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>gsmooth.SetDimensionality(3);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>gsmooth.Update();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>vtkImageShrink3D mask = new vtkImageShrink3D();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>mask.SetInput(gsmoouth.GetOutput());</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>mask.SetShrinkFactors(5, 5, 5);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>vtkMarchingCubes iso = new vtkMarchingCubes();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>iso.SetInput(mask.GetOutput());</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>iso.SetValue(0, 150); //(0,150)</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>iso.ComputeGradientsOn();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>iso.ComputeScalarsOff();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>iso.SetLocator(locator);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>vtkVectorNorm gradient = new vtkVectorNorm();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>gradient.SetInput(iso.GetOutput());</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>vtkDataSetMapper isoMapper = new vtkDataSetMapper();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>isoMapper.SetInput(gradient.GetOutput());</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>isoMapper.ScalarVisibilityOn();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>isoMapper.SetScalarRange(0, 600); //(0,300)</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>isoMapper.ImmediateModeRenderingOn();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>vtkActor isoActor = new vtkActor();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>isoActor.SetMapper(isoMapper);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>isoActor.GetProperty().SetColor(100, 100, 100);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>vtkOutlineFilter outline = new vtkOutlineFilter();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>outline.SetInput(reader.GetOutput());</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>vtkPolyDataMapper outlineMapper = new vtkPolyDataMapper();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>outlineMapper.SetInput(outline.GetOutput());</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>vtkActor outlineActor = new vtkActor();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>outlineActor.SetMapper(outlineMapper);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>ren1.AddActor(outlineActor);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>ren1.AddActor(isoActor);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>ren1.AddLight(lgt);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>renWin.SetSize(1000, 1000);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>ren1.SetBackground(0, 0, 0.4);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>vtkCamera cam1 = ren1.GetActiveCamera();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>cam1.Elevation(600);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>cam1.SetViewUp(0, 0, 1.0);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>cam1.Zoom(0.5);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>cam1.SetDistance(300);</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>lgt.SetPosition(cam1.GetPosition());</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>lgt.SetFocalPoint(cam1.GetFocalPoint());</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>ren1.ResetCameraClippingRange();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"> </font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>renWin.Render();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri"><span style="mso-spacerun: yes"> </span>iren.Start();</font></font></span></p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font face="Calibri" size="3"></font></span> </p>
<p class="MsoNoSpacing" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="mso-ansi-language: EN-US"><font size="3"><font face="Calibri">Thank Elena and Amy for the reply.</font></font></span></p></div>